Aktivitas Antibakteri Dan Antimotilitas Eugenol Terhadap Bakteri Escherichia coli

Isnawati, Nur Ilmi and , Ratna Yuliani, M.Biotech., Ph.D (2026) Aktivitas Antibakteri Dan Antimotilitas Eugenol Terhadap Bakteri Escherichia coli. Skripsi thesis, Universitas Muhammadiyah Surakarta.

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Abstract

Antibiotic resistance is a major global health problem, with millions of associated deaths reported in 2019, and Escherichia coli is a Gram-negative pathogen of concern due to its ability to cause various infections and its increasing resistance to multiple antibiotics. Moreover, antibiotic-resistant E. coli is listed as a high-priority pathogen in the WHO Bacterial Priority Pathogens List 2024. These challenges highlight the urgent need for novel antibacterial candidates, including natural compounds such as eugenol, a major constituent of clove essential oil, which has been reported to possess antibacterial activity and may affect bacterial virulence-related traits. This study aimed to evaluate the antibacterial and antimotility activities of eugenol against E. coli. Antibacterial activity was assessed using a resazurin-based microdilution assay in a 96-well microplate to determine the Minimum Inhibitory Concentration (MIC), followed by agar subculture to determine the Minimum Bactericidal Concentration (MBC). Antimotility activity was evaluated using anti-swimming and anti-swarming assays in 6-well plates. The resazurin microdilution test showed an MIC of eugenol at 1 mg/mL, while the MBC was >1 mg/mL. In antimotility assays, eugenol reduced E. coli motility, with swimming inhibition of 32.9% at 0.25 mg/mL and 100% at 0.5 mg/mL and 1 mg/mL. Eugenol also inhibited swarming motility, with inhibition of 8.3% at 0.25 mg/mL and 100% at 0.5 mg/mL and 1 mg/mL. These findings indicate that eugenol has potential as an antibacterial and antimotility agent against E. coli.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	antibacterial, Escherichia coli, eugenol, motility, resazurin microdilution
Subjects:	R Medicine > Pharmacy and materia medica
Divisions:	Fakultas Farmasi > S1 Farmasi
Depositing User:	NUR ILMI ISNAWATI
Date Deposited:	07 May 2026 03:01
Last Modified:	07 May 2026 03:01
URI:	http://eprints.ums.ac.id/id/eprint/144555

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