Uji Aktivitas Antikanker Kombinasi Ekstrak Etanol Umbi Bawang Dayak (Eleutherine Americana Merr.) Dan Biji Sirsak (Annona Muricata) Dengan Metotreksat Terhadap Sel T47d

Nuur Anisa Putri, Erika and , Dr. Haryoto, M.Sc (2018) Uji Aktivitas Antikanker Kombinasi Ekstrak Etanol Umbi Bawang Dayak (Eleutherine Americana Merr.) Dan Biji Sirsak (Annona Muricata) Dengan Metotreksat Terhadap Sel T47d. Skripsi thesis, Universitas Muhammadiyah Surakarta.

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Abstract

Breast cancer nowadays is the mostly responsible types of cancer to cause the mortality in the world after lungs, stomach dan liver cancer. Breast cancer as the number one factor to cause death in cancer with number of incidents 43,1% in Indonesia. This research is conducted to determine anticancer activity in combination of ethanolic extract of dayak onion’s bulbs (Eleutherine Americana Merr.) and sousop seed (Annona muricata) with methotrexate on T47D cell. The method used for examined cytotoxic activity is MTT-Assay. The living cells will produced a purple fomazan’s crystal and it intensity have a correlation with the numbers of living cells. This test used various of concentration of each sample in a row such as 500; 250; and 125 μg/mL for dayak onion’s bulbs, 250; 125 and 62,5 μg/mL for soursop seeds, sothen 50; 25; 12,5 and 6,25 μg/mL for methotrexate. IC50 values for dayak onion’s bulbs is 251,18 μg/mL, soursop with IC50 as 67,61 μg/mL and 16,22 μg/mL for the IC50 of methotrexate. The values of IC50 then used to determined the concentration for combination test. Combination test used variety of concentration which are ½ IC50, ¼ IC50 dan 1/8 IC50 with the IC50 that has been determined before. This combination use single extract with cytotoxic drug; methotrexate. Combination test of ethanol extract of soursop with MTX showed synergistic values with 35 μg/mL of soursop concentration and 2; 4 μg/mL of MTX giving Combination Index (CI) values 0,71 and 0,39 in a row. Whereas combination between dayak onion’s bulb with MTX giving antagonist activity.

Item Type:	Karya ilmiah (Skripsi)
Uncontrolled Keywords:	Dayak onions, soursop seeds, IC50, combination index
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Farmasi
Depositing User:	ERIKA NUUR ANISA PUTRI
Date Deposited:	06 Feb 2018 06:14
Last Modified:	06 Feb 2018 06:14
URI:	http://eprints.ums.ac.id/id/eprint/59241

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