SUBEKTI, ASRI (2009) UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN WARU LANDAK (Hibiscus mutabilis L.) TERHADAP Staphylococcus aureus DAN Escherichia coli SERTA BRINE SHRIMP LETHALITY TEST. Skripsi thesis, Univerversitas Muhammadiyah Surakarta.
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Abstract
Tanaman waru landak adalah salah satu tanaman obat yang berdasarkan pengalaman digunakan sebagai antimikroba dan antikanker. Penelitian ini dilakukan dengan tujuan untuk mengetahui toksisitas ekstrak etanol daun waru landak (Hibiscus mutabilis L.) terhadap Artemia salina Leach dan aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli serta mengetahui metabolit sekunder yang terkandung didalamnya. Ekstrak etanol 70% daun waru landak dibuat dengan cara maserasi. Ekstrak diuji aktivitas antibakterinya menggunakan metode dilusi padat untuk mengetahui kadar bunuh minimalnya. Untuk uji toksisitasnya menggunakan metode Brine Shrimp Lethality Test (BST). Data diperoleh dengan menghitung jumlah larva yang mati setelah 24 jam. Persen kematian digunakan untuk menghitung harga LC50 (Lethal Concentration 50) dengan metode analisis probit. Untuk mengetahui kandungan senyawa dari ekstrak digunakan kromatografi lapis tipis menggunakan fase diam silika gel F254 dan fase gerak kloroform : metanol ( 9,5 : 0,5 ) dengan deteksi sinar UV 254 nm dan 365 nm dan pereaksi semprot FeCl3, uap amonia, sitroborat, Liebermann-Bourchard, dan Dragendorff. Hasil uji toksisitas menunjukkan harga LC50 320,45 µg/ml, ini menunjukkan bahwa ekstrak etanol daun waru landak memiliki efek toksik yang rendah. Untuk uji aktivitas antibakteri menunjukkan kadar bunuh minimal terhadap Staphylococcus aureus 3% b/v dan Escherichia coli 2,5% b/v. Hasil analisis kromatografi lapis tipis menunjukkan bahwa ekstrak etanol daun waru landak mengandung senyawa polifenol, flavonoid, terpenoid, dan alkaloid. Kata kunci : Hibiscus mutabilis L., Antibakteri, Staphylococcus aureus, Escherichia coli, Brine Shrimp Lethality Test (BST), kromatografi lapis tipis. ANTIBACTERIA ACTIVITY TEST OF ETHANOL EXTRACT FROM WARU LANDAK LEAVES (Hibiscus mutabilis L.) TO Staphylococcus aureus AND Escherichia coli AND ALSO BRINE SHRIMP LETHALITY TEST ABSTRACT Crop of Waru landak is one of drug crop which are pursuant to experience used as antimicrobial and anticancer. This research done as a mean to know the toxicity of extract of ethanol of leaf of waru landak (Hibiscus mutabilis L.) to Artemia salina Leach and activity antibacterial to Staphylococcus aureus and Escherichia coli and also know the secondary metabolite which consisted in it. Extract ethanol 70% of leaf of waru landak made by maceration. Extract tested the activity of antibacterial, there was used the solid method dilution to knowing the minimum rate kill. To test the toxicity used the method of Brine Shrimp Lethality Test (BST). Data obtained by counting dead larva after 24 hour. Percentage of death used to calculate LC50 (Lethal Concentration 50) with the probit method analyses. To know the compound content from extract used thin layer chromatography was used the stationary phase by silica gel of F254 and mobile phase the chloroform : methanol (9,5 : 0,5) detectedly in UV 254 nm and 365 nm and with spray of the Fecl3, condense the ammonia, citroborat, Liebermann-Bourchard, and Dragendorff. Result of toxicities test showed the LC50 320,45 µg / ml, this indicate that the extract of ethanol of leaf of waru landak had the low toxic effect. To test the activity antibacterial showed the minimum rate kill to Staphylococcus aureus 3% b/v and Escherichia coli 2,5% b/v. Result of analysis of chromatography lamella indicate that the extract of ethanol of leaf of waru landak were contain the compound polifenol, flavonoid, terpenoid, and alkaloid. Keywords : Hibiscus mutabilis L., Antibacterial Test Activity, Staphylococcus aureus, Escherichia coli, Brine Shrimp Lethality Test (BST), thin layer chromatograpy.
| Item Type: | Karya ilmiah (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Hibiscus mutabilis L., Antibacterial Test Activity, Staphylococcus aureus, Escherichia coli, Brine Shrimp Lethality Test (BST), thin layer chromatograpy. |
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisions: | Fakultas Farmasi > Farmasi |
| Depositing User: | Mrs Esti Handayani |
| Date Deposited: | 28 Jan 2010 07:45 |
| Last Modified: | 15 Nov 2010 18:06 |
| URI: | http://eprints.ums.ac.id/id/eprint/5839 |
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