Deteksi Apoptosis Dengan Metode Flowcytometry Annexin-V Pada Model Sel Ligamen Periodontal

Jasamania HR, Jesyta and , drg. Mahmud Kholifa, MDSc (2021) Deteksi Apoptosis Dengan Metode Flowcytometry Annexin-V Pada Model Sel Ligamen Periodontal. Skripsi thesis, Universitas Muhammadiyah Surakarta.

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Abstract

Introduction: Cells experience death by two mechanisms, namely apoptosis and necrosis. In physiological conditions, apoptosis is very important for cell turnover in tissues during normal development. Apoptosis is also considered an essential component for immune system function, hormone-dependent atrophy, embryonic development and chemical-induced cell death. In normal cells, periodontal ligament fibroblasts can be detected in vitro apoptosis by using Annexin v flowcytometry laboratory tests. Purpose: This literature review is to determine the success of the Annexin V flow cytometry method in detecting apoptosis in periodontal ligament cells. Research method: Flowcytometry in principle uses the Annexin V test which binds phosphatidylserine on the surface of cells experiencing apoptosis. Annexin v is used as a marker to detect apoptotic cells. Propidium iodide is used to differentiate living cells, apoptosis, and necrosis. Conclusion: The Flow Cytometry test method is a sophisticated technology that provides a quick overview, is able to take quantitative measurements, and is reliable for detecting apoptosis. The results of staining on cells obtained 4 kinds of colors, namely green indicates living cells, yellow indicates early apoptosis, pink indicates late apoptosis, and red indicates necrosis.

Item Type:	Karya ilmiah (Skripsi)
Uncontrolled Keywords:	Apoptosis; Flow Cytometry; Annexin V; Periodontal Ligament Fibroblasts
Subjects:	R Medicine > R Medicine (General)
Divisions:	Fakultas Kedokteran Gigi > Kedokteran Gigi
Depositing User:	JESYTA JASAMANIA HR
Date Deposited:	16 Aug 2021 03:08
Last Modified:	16 Aug 2021 03:09
URI:	http://eprints.ums.ac.id/id/eprint/93419

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