Uji Karakteristik Enzim Endo-Β-1,4-Glukanase, Ekso-Β-1,4-Glukanase Dan Β-Glukosidase Pada Fungi Aspergillus Niger Dan Kinetika Enzim Campuran

Karintiana, Dea and , Hamid Abdillah, S.T., M.T (2017) Uji Karakteristik Enzim Endo-Β-1,4-Glukanase, Ekso-Β-1,4-Glukanase Dan Β-Glukosidase Pada Fungi Aspergillus Niger Dan Kinetika Enzim Campuran. Skripsi thesis, Universitas Muhammadiyah Surakarta.

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Abstract

Cellulase is a group of enzymes that break down cellulose into glucose monomers obtained from endo-β-1,4-glucanase enzymes, ekso-β-1,4-glucanase and β-glucosidase produced by fungi or yeast. The purpose of this research is to know the characteristics of endo-β-1,4-glucanase enzyme, ekso-β-1,4-glucanase and β-glucosidase produced by Aspergillus niger L-76 and Aspergillus niger 6114 with substrate variation is CMC, Avicel , and Arbutin and Aspergillus niger characteristics test to get the highest productivity of Aspergillus niger L-76 which will be combined with yeast obtained by Debaryomycess hansenni. Aspergillus niger l-76 strain that has content in the highest 4 days enzyme production is 0.0592 units / ml which will be mixed with Debaryomycess hansenni is 0.2650212 units / ml to test the reaction kinetics of fungi and yeast mixture with a ratio of 1: 1.1 : 2, and 2: 1. The highest mixed enzyme produced in a 2: 1 ratio with CMC substrate variation of 0.3; 0.4; 0.5; 0.6; 0.7; 0.8; 0.9; and 1 g / L has an enzyme Vmax enzyme parameter of 0.200080032 g / L and Km of 1,589636 L / g.

Item Type: Karya ilmiah (Skripsi)
Uncontrolled Keywords: Fungi Aspergillus niger, Cellulase, CMC, Avicel, Arbutin.
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
Divisions: Fakultas Teknik > Teknik Kimia
Depositing User: DEA APRILIYANTIKA K
Date Deposited: 23 Jul 2018 10:29
Last Modified: 23 Jul 2018 10:30
URI: http://eprints.ums.ac.id/id/eprint/63843

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