Uji Karakteristik Enzim ß-1,4-Glukosidase Pada Berbagai Yeast Serta Kinetika Reaksi Enzim Campuran

Rahmawati, Elox Yuniar and , Hamid Abdillah, S.T., M.T. (2018) Uji Karakteristik Enzim ß-1,4-Glukosidase Pada Berbagai Yeast Serta Kinetika Reaksi Enzim Campuran. Skripsi thesis, Universitas Muhammadiyah Surakarta.

	PDF (Naskah Publikasi) naskah publikasi-328.pdf Download (2MB)
	PDF (Pernyataan Publikasi Ilmiah) Surat Pernyataan Naskah Publikasi.pdf Restricted to Repository staff only Download (246kB) | Request a copy

Abstract

Cellulase is a reduced enzyme synthesized from microorganisms grown in cellulose medium. Cellulase and β-glucosidase enzymes are present in various types of bacteria, fungi and yeasts. Strains of yeast Debaryomyces Hansenii and Debaryomyces Polymorphus show high β-glucosidase activity while Aspergillus Niger fungi produce high cellulase activity and less-glucosidase. Cellulase enzyme can hydrolyze cellulose into glucose by fermentation. In this study, enzyme mixing of yeast fermentation and fermentation of fungi was done. Strain of Debaryomyces Hansenii has the highest β-glucosidase content in 4 days production of enzyme 0,2650212 units / ml mixed with Aspergillus Niger L-76 0,0592 units / ml fungi for kinetics test of mixed enzyme reaction with yeast ratio: fungi = 1: 1, 1: 2 and 2: 1. The highest mixed enzymes were produced at a 2: 1 ratio and had the enzyme kinetic parameters of Vmax of 0.200080032 g / L and Km 1.589636L / g using a variation of CMC substrate concentration of 0.3; 0.4; 0.5; 0.6; 0.7; 0.8; 0.9; and 1g / L.

Item Type:	Karya ilmiah (Skripsi)
Uncontrolled Keywords:	Enzim Selulase, Yeast, Congo Red, Fermentasi, Kinetika Reaksi
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry Q Science > QR Microbiology
Divisions:	Fakultas Teknik > Teknik Kimia
Depositing User:	ELOX YUNIAR RAHMAWATI
Date Deposited:	10 Apr 2018 06:55
Last Modified:	10 Apr 2018 06:55
URI:	http://eprints.ums.ac.id/id/eprint/61777

Actions (login required)

View Item