Antibacterial Activity of Sugarcane Endophytic Fungi In Staphylococcus Aureus ATCC 25923 And MRSA

Ain, Vania Qurratu and , dr. Iin Novita Nurhidayati Mahmuda, M.Sc., Sp.PD, FINASIM (2025) Antibacterial Activity of Sugarcane Endophytic Fungi In Staphylococcus Aureus ATCC 25923 And MRSA. Skripsi thesis, Universitas Muhammadiyah Surakarta.

	PDF (Naskah Publikasi) J500210166-NASKAH PUBLIKASI-ICMB ISETH.pdf Restricted to Repository staff only Download (369kB)
	PDF (Surat Pernyataan Publikasi) J500210166-Surat Pernyataan Publikasi-TTD.pdf Restricted to Repository staff only Download (230kB)
	PDF (LoA) J500210166-LoA.pdf Restricted to Repository staff only Download (293kB)

Abstract

Background: Antibacterial resistance is a critical global health issue, causing 4.95 million deaths in 2019 and projected to increase healthcare costs by $1 trillion by 2050. Staphylococcus aureus, including methicillin-resistant Staphylococcus aureus (MRSA), is a major cause of nosocomial infections. Aim: This study aimed to investigate the antibacterial activity of sugarcane (Saccharum officinarum L.) endophytic fungal isolates against Staphylococcus aureus ATCC 25923 and MRSA. Methodology: This experimental study was conducted using a posttest-only control group design. Endophytic fungi were isolated from the roots, stems, and leaves of sugarcane, fermented, and extracted secondary metabolites with ethyl acetate solvent. The antibacterial activity was tested using the Kirby-Bauer disk diffusion method with concentrations of 25%, 50%, and 100%. Results: The identified endophytic fungi, Aspergillus sp., demonstrated significant antibacterial activity. At a 100% concentration, the inhibition zone diameter was 14.6 mm for S. aureus ATCC 25923 and 11.6 mm for MRSA. Kruskal-Wallis and Mann-Whitney statistical tests showed significant differences between groups. Discussion: This antibacterial potential is related to the secondary metabolites of the fungus, such as phenolic compounds, flavonoids, and tannins, which act by damaging the bacterial membrane and inhibiting important enzymes. Conclusion: Sugarcane endophytic fungal isolates have antibacterial activity against Staphylococcus aureus ATCC 25923 and MRSA, with the highest effectiveness at 100% concentration. Further research is needed for identification of active compounds and in vivo tests.

Item Type:	Thesis (Skripsi)
Additional Information:	Artikel sudah di upload di ICMB dan ISETH tahun 2024
Uncontrolled Keywords:	Antibacterial activity, Endophytic fungal isolates, Sugarcane, Staphylococcus aureus ATCC 25923, MRSA
Subjects:	R Medicine > General Medicine R Medicine > Pharmacy and materia medica > Biological Pharmacy R Medicine > Pharmacy and materia medica > Ekstrak Tumbuhan Obat
Divisions:	Fakultas Kedokteran > S1 Kedokteran
Depositing User:	VANIA QURRATU AIN
Date Deposited:	16 Jan 2025 04:11
Last Modified:	15 Mar 2025 03:35
URI:	http://eprints.ums.ac.id/id/eprint/130020

Actions (login required)

View Item