Uji Aktivitas Antivirus Ekstrak Etanol Daun Kemangi (Ocimi Basilica F. Citrate Folium) Dengan Target Virus Newcastle Disease

Nurmianigsih, Septiana Fadhila and , apt. Azis Saifudin,Ph.D (2023) Uji Aktivitas Antivirus Ekstrak Etanol Daun Kemangi (Ocimi Basilica F. Citrate Folium) Dengan Target Virus Newcastle Disease. Skripsi thesis, Universitas Muhammadiyah Surakarta.

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Basil leaves (Ocimi basilica f. citrate folium) are one of the many natural ingredients that have been researched for their contents and uses. This plant is reported to have benefits ranging from anti-inflammatory, antiviral and antibacterial. The Newcastle Disease virus model was chosen as a strategy in searching for an antiviral agent for COVID-19 because both are RNA viruses that both attack the respiratory system. This study aims to determine the antiviral activity contained in the leaves of the basil plant (Ocimi basilica f. citrate folium) against viral agents in the Newcastle Disease model. Basil leaves are extracted using the maceration method. The resulting extract was inoculated into the allantois of 9-10 day old embryonated chicken eggs (TAB) and incubated for 3 days. A total of 16 eggs were divided into 6 groups (1 µg/mL, 10 µg/mL, 100 µg/mL, positive control antiviral compound ethyl 4-(4-hydroxy-3- methoxyphenyl)-6-methyl-2-oxo-1, 2,3,4-tetrahydropyrimide-5-carboxylate, virus control, and negative control). During the incubation process, eggs are checked every day to observe embryo death. After incubation again for 3 days, a hemagglutination test was carried out. Hemagglutination test results showing antiviral activity were marked by the formation of a dot in the center of the microplate well. The results of research on the antiviral activity of the ethanol extract of basil leaves (Ocimi basilica f. citrate folium) did not show antiviral activity against the Newcastle Disease virus at concentrations of 1 µg/mL, 10 µg/mL, or 100 µg/mL, whereas in the positive control the antiviral compound ethyl 4-(4-hydroxy-3- methoxyphenyl)-6-methyl-2-oxo-1,2,3,4-tetrahydropyrimide-5-carboxylate has antiviral activity of 49.95% at a concentration of 100 µg/mL and 99, 89% at a concentration of 10 µg/mL.

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: Antivirus, Newcastle Disease, Ocimum basilicum, Sweet Basil Leaves.
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Farmasi > Farmasi
Date Deposited: 02 Nov 2023 01:32
Last Modified: 02 Nov 2023 01:32
URI: http://eprints.ums.ac.id/id/eprint/117692

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