Uji Aktivitas Sitotoksik Ekstrak Metanol, Fraksi Butanol, Fraksi Etil Asetat, Dan Fraksi Kloroform Buah Kemukus (Piper Cubeba) Terhadap Sel Kanker T47D

Mulyawati, Sita and , Azis Saifudin, Ph.D., Apt. (2018) Uji Aktivitas Sitotoksik Ekstrak Metanol, Fraksi Butanol, Fraksi Etil Asetat, Dan Fraksi Kloroform Buah Kemukus (Piper Cubeba) Terhadap Sel Kanker T47D. Skripsi thesis, Universitas Muhammadiyah Surakarta.

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Abstract

Breast cancer is one type of cancer that causes major deaths in the female population in Indonesia. Most cancer therapies cause unwanted effects for patients so many patients are turning to treatment by using materials derived from nature. Methanol extract of the kemukus fruit (Piper cubeba) is known to have cytotoxic activity against breast cancer cells MCF-7, MDA-MB-231, MDA-MB-468. Several previous studies no research has been done on the kemukus fruit of T47D cancer cells, so the purpose of this research is to know cytotoxic activity in methanol extract, butanol fraction, ethyl acetate fraction, and chloroform fraction of kemukus fruit against T47D cancer cells. The maceration method with methanol was selected for extracting kemukus fruit (Piper cubeba). The cytotoxic activity test was performed with an MTT assay. Extracts and fractions that have less IC50 values were tested the content of compounds with TLC (Thin Layer Chromatography).. The result of cytotoxic activity test showed that IC50 methanol extract and chloroform fraction of kemukus fruit (Piper cubeba) were 134,95 μg / mL and 225.3 μg / mL, respectively butanol fraction and ethyl acetate fraction show that did not have cytotoxic activity. The result of TLC showed that methanol extract and chloroform fraction contain secondary metabolite compound of alkaloids, lignans, and terpenoids.

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: Piper cubeba, MTT assay, Cytotoxic, T47D
Subjects: R Medicine > R Medicine (General)
Divisions: Fakultas Farmasi > Farmasi
Depositing User: Sita Mulyawati
Date Deposited: 30 Jan 2018 03:09
Last Modified: 30 Jan 2018 03:09
URI: http://eprints.ums.ac.id/id/eprint/58555

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