Uji Aktifitas Sitotoksik Ekstrak Etanol, Fraksi Polar, Semipolar, dan Nonpolar Herba Kitolod (Isotoma longiflora (L.) C. Presl.) terhadap Sel MCF-7

Hapsari, Atikah and , Dr. Haryoto, M.Sc. (2016) Uji Aktifitas Sitotoksik Ekstrak Etanol, Fraksi Polar, Semipolar, dan Nonpolar Herba Kitolod (Isotoma longiflora (L.) C. Presl.) terhadap Sel MCF-7. Skripsi thesis, Universitas Muhammadiyah Surakarta.

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Abstract

Breast cancer is a major cancer suffered and the most cause of death by women in the world. The existing drugs of anticancer are not selective, disturbing the growth of normal cells. It encourages researchers to explore a chemopreventive agent from the nature materials. This study was conducted to determine the cytotoxic activity of ethanol extract, polar fractions, semipolar, and nonpolar herb kitolod (Isotoma longiflora (L) Presl.) on MCF-7 cells. First, herb kitolod was dried, refined, and sieved using mesh number 40 and then macerated using 96% ethanol for 72 hours. Phytochemical screening was conducted with TLC using GF254 silica as stationary phase, n-hexane: ethyl acetate (7: 3) as mobile phase, then the result was visualized on UV 254nm, 366nm, and visible light. Anisaldehid, dragendroff, sitoborat, and FeCl3 reagents spray were used as visualizer of TLC spot results. Fractination using KCV method, then cytotoxic test using MTT assay. A result obtained, the ethanol extract of herb kitolod positively contains flavonoids, phenolics, alkaloids, terpenoids, saponins, steroids and tannins. IC50 value of the ethanol extract, polar fractions, semipolar, and nonpolar are 521,054; 213,294; 499,947; and 239,431 µg/mL. This study showed that the ethanol extract, polar fraction, semipolar, and nonpolar of herb kitolod had moderate cytotoxic activity on MCF-7 cells.

Item Type:	Karya ilmiah (Skripsi)
Uncontrolled Keywords:	Sitotoksik, Kitolod, Fraksi, MTT assay, Sel MCF-7
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Farmasi > Farmasi
Depositing User:	ATIKAH HAPSARI
Date Deposited:	20 Jan 2017 09:35
Last Modified:	20 Jan 2017 09:35
URI:	http://eprints.ums.ac.id/id/eprint/48514

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